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将抗逆基因ThIPK2转入东农50,得到稳定遗传株系,将T4代株系种子正常播种,将转基因植株与对照东农50的生育期、株高、主茎节数、分枝数、主茎荚数、分枝荚数、单株粒数等性状进行比较,研究ThIPK2基因对大豆农艺性状方面的影响。结果表明,在正常条件下,转基因株系和对照植株相比,生育期并没有改变,而株高、主茎荚数、分枝荚数、分枝数和单株粒数农艺性状呈显著或极显著提高,这表明ThIPK2基因对提高大豆产量有利。干旱胁迫导致大豆叶片各项光合生理指标下降;但转ThIPK2基因大豆的各项光合生理指标高于非转基因对照。经干旱胁迫后,转基因植株与对照相比积累了更多的干物质。
The resistant gene ThIPK2 was transferred into Dongnong 50 to obtain a stable genetic line. The seeds of the T4 generation were planted normally, and the growth period, plant height, number of stems, number of branches, The number of main stem pods, the number of pods and the number of grains per plant were compared to study the influence of ThIPK2 gene on the agronomic characters of soybean. The results showed that under normal conditions, the growth of transgenic lines did not change compared with the control plants, but the plant height, the number of main stem pods, branch pods, branch number and agronomic characters per plant were significant or Significantly increased, indicating that the ThIPK2 gene is beneficial for increasing soybean yield. Drought stress led to the decrease of all photosynthetic physiological indexes of soybean leaves, but photosynthetic physiological indexes of transgenic ThIPK2 soybean were higher than non-transgenic control. After drought stress, the transgenic plants accumulated more dry matter than the control.