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采用安全简便的方法对人体精子的基础钙(~(45)Ca~(2+))吸收问题进行了分析研究。结果发现~(45)Ca~(2+)的吸收呈时间依赖性过程,其最高吸收值为0.23nmol/mg蛋白质,这是在实验开始后400秒时达到的。即使将钾的浓度从0.7mmol增至50mmol(并降低相应的氯化钠浓度)也不能促进~(45)Ca~(2+)吸收。 ~(45)Ca~(2+)吸收以剂量依赖性方式被异搏定、三氟拉嗪和抗霉素A抑制,其最大抑制浓度的半数值分别是130μmol、7μmol和50nmol。 氯化钾和钙通道阻滞剂异搏定20μmol的去极化浓度不能改变~(45)Ca~(2+)的转运,但在高于20μmol异搏定浓度情况下,发生了剂量依赖性钙吸收抑制。这就很难用通道阻滞剂的门控电压来解释。事
The safe and convenient method was used to analyze the absorption of calcium (~ (45) Ca ~ (2+)) in human sperm. The results showed that the uptake of ~ (45) Ca ~ (2+) was time-dependent and its maximum absorption was 0.23 nmol / mg protein, which was reached at 400 seconds after the start of the experiment. Even if the concentration of potassium is increased from 0.7 mmol to 50 mmol (and the corresponding sodium chloride concentration is lowered), the absorption of ~ (45) Ca 2+ can not be promoted. ~ (45) Ca ~ (2+) uptake was inhibited by verapamil, trifluoperazine and antimycin A in a dose-dependent manner. The maximal inhibitory concentrations of ~ (45) were 130μmol, 7μmol and 50nmol, respectively. Weak potassium chloride and calcium channel blockers verapamil 20μmol depolarization concentration can not change the ~ (45) Ca ~ (2+) transport, but higher than 20μmol verapamil concentration, a dose-dependent Calcium absorption inhibition. This is difficult to explain with the gate-blocker’s gate voltage. thing