论文部分内容阅读
经一年的初步试验研究表明,用利粒菠萝冠芽的一个整尖作外值体进行组织培养,经连续四次继代增殖,年繁殖数可达85万倍以上,试管繁殖可用于剥粒菠萝的快速繁殖.继代增殖过程中,液体振荡培养比液体静置培养增殖率平均高3.7倍;应用低浓度6—BA和K,并与低浓度的NAA、IAA配合,是有利提高增殖芽的数量和质量的较佳培养基;取用无菌苗和芽作继代增殖材料,其苗比芽的继代增殖率高5.7～27.3%.
After a year of preliminary experimental studies have shown that the use of anaphase pineapple crown buds as a whole value of the outer tissue for tissue culture, after four successive subcultures, the annual breeding number of up to 850,000 times more than the tube can be used for breeding stripping In the process of subculture, liquid proliferation was 3.7 times higher than that of liquid static culture. The application of low concentration of 6-BA and K with low concentration of NAA and IAA was beneficial to increase proliferation The number of buds and the quality of the best medium; take sterile seedlings and shoots for the generation of proliferation of materials, the seedling bud than the secondary proliferation rate of 5.7 to 27.3%.