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目的 :探讨白细胞介素 13 (IL 13 )在小儿原发性肾病综合征 (NS)中的变化及其与病理形态之间的关系。 方法 :应用逆转录 多聚酶链反应 (RT PCR)及双抗体夹心ELISA法检测了NS患儿外周血单个核细胞IL 13mRNA及蛋白水平的变化 ,并分析其与病理形态改变的关系。 结果 :原发性NS患儿肾病期IL 13mRNA与蛋白水平均显著高于正常对照组 ( 0 4 6± 0 15vs 0 3 6± 0 16,P <0 0 5和 4 8 2 0± 15 90ng/Lvs 3 5 2 2± 4 4 2ng/L ,P <0 0 1) ,缓解期与正常对照组相比差异不显著。IL 13表达与患儿肾脏病理形态改变存在一定的关系 ,以系膜增生性肾小球肾炎和IgA肾病最为明显。 结论 :Th1/Th2细胞免疫功能失衡在原发性NS发病过程中发挥着重要作用 ,IL 13在体内可能具有一定的抗炎作用
Objective: To investigate the change of interleukin-13 (IL-13) in children with primary nephrotic syndrome (NS) and its relationship with pathological morphology. Methods: The levels of IL - 13 mRNA and protein in peripheral blood mononuclear cells of children with NS were measured by reverse transcriptase - polymerase chain reaction (RT PCR) and double antibody sandwich enzyme - linked immunosorbent assay (ELISA). The relationship between IL - 13 mRNA and protein levels was also analyzed. Results: The levels of IL-13 mRNA and protein in nephropathy patients with primary NS were significantly higher than those in normal controls (0 46 ± 0 15 vs 0 36 ± 0 16, P 0 05 and 48 20 ± 15 90 ng / L 3 5 2 2 ± 4 4 2ng / L, P 0 01). There was no significant difference between the two groups in remission time and control group. There is a certain relationship between the expression of IL-13 and pathological changes of renal kidneys, most notably mesangial proliferative glomerulonephritis and IgA nephropathy. Conclusion: The imbalance of immune function of Th1 / Th2 cells plays an important role in the pathogenesis of primary NS. IL 13 may have some anti-inflammatory effects in vivo