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目的对小连翘(Hyperioums Sampsonii Hance.)内生放线菌YBL-02菌株的进行分类、发酵及产物的提取和初步鉴定。方法根据形态学,培养特征等初步鉴定YBL-02菌株,并采用薄层色谱和红外光谱等对YBL-02发酵产物进行分离和初步鉴定。结果根据菌株的形态学,培养特征等初步将YBL-02菌株归属为链霉菌属。对YBL-02菌株发酵产物进行分离纯化,最后得到具有活性的片状白色结晶体,测其熔点为117～119℃。对该物质进行红外光谱分析后可得出为一种酰胺类化合物。结论YBL-02菌株是一种链霉菌,其发酵产物中活性成分为一种酰胺类化合物。
Objective To classify, ferment, extract and identify the endophytic actinomycetes YBL-02 from Hyperioums Sampsonii Hance. Methods The strain YBL-02 was initially identified according to morphology and culture characteristics. The fermentation products of YBL-02 were separated and identified by TLC and FTIR. Results The strain YBL-02 was initially classified as Streptomyces according to the morphology and culture characteristics of the strains. YBL-02 strains were isolated and purified fermentation products, and finally get active flaky white crystals, measuring the melting point of 117 ~ 119 ℃. Infrared spectroscopy of the material can be obtained as an amide compound. Conclusion YBL-02 strain is a kind of Streptomyces, the active ingredient in the fermentation product is an amide compound.