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提出了增效联磺片中磺胺甲基异唑(SMZ)、磺胺嘧啶(SD)及甲氧节胺嘧啶(TMP)三组分薄层色谱同时测定的新方法。该法以水饱和的乙酸乙酯-氯仿(2:1)的混合溶剂为展开剂,在硅胶GF_(254)板上将被测各组分分离后,直接进行薄层色谱扫描测定,操作简便、快速,灵敏度高,重现性好。
A new method for the simultaneous determination of sulfamethoxazole (SMZ), sulfadiazine (SD) and trimethoprim (TMP) by TLC in the synergistic combination is proposed. This method was carried out by thin layer chromatographic scanning with the mixture of ethyl acetate-chloroform (2: 1) as developing solvent and silica gel GF_ (254) Fast, sensitive and reproducible.