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目的:采用高效液相色谱法测定和丹舒胶囊中黄芪甲苷含量。方法:采用DiamonsilTM(4.6mm×250mm,5μm)色谱柱;乙腈-水(36:64)为流动相;流速1.0mL.min-1;漂移管温度:105℃;载气流速:2.7L/min。结果:黄芪甲苷进样量在1.21~12.19μg范围内呈现良好的线性关系,平均加样回收率为99.33%,RSD为1.03%(n=9)。结论:本法简便、准确,可作为该制剂的质量控制方法。
Objective: To determine the content of Astragaloside IV in Danshu capsule by HPLC. Methods: DiamonsilTM (4.6mm × 250mm, 5μm) column was used. Acetonitrile-water (36:64) was used as the mobile phase. The flow rate was 1.0mL.min-1. The temperature of the drift tube was 105 ℃. The carrier gas flow rate was 2.7L / min . Results: Astragaloside injection showed a good linearity in the range of 1.21-12.19 μg with an average recovery of 99.33% and a RSD of 1.03% (n = 9). Conclusion: This method is simple and accurate and can be used as the quality control method of this preparation.