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Objective:To study the antitumor effects and associated mechanisms of extract of the Smilax china L rhizome(SCR) on ovarian cancer cells.Methods:Ovarian cancer cells A2780 were treated with different concentrations of SCR extract(SCRE),and compared with controls.Effects on cell growth were evaluated by cell counting kit-8(CCK-8) assay;proliferation effects by EdU incorporation assay;cell cycle by propidium iodide staining;apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide;cellular distribution of nuclear factor- k B(NF- κB) by immunofluorescence;protein levels of NF- κB,caspase-3,poly-adenosine diphosphate(ADP)-ribose polymerase(PARP),Bcl-2-associated X protein(Bax),cellular inhibitor of apoptosis(cIAP)-1,anti-X-linked inhibitor of apoptosis protein(XIAP),B-cell lymphoma-extra large(Bcl-XL),B-cell lymphoma-2(Bcl-2) and AKT by Western blotting;and effects of SCRE combined with cisplatin or adriamycin on A2780 cells by CCK-8 assay.Results:SCRE suppressed A2780 cell proliferation in a dose-dependent manner(P<0.05,P<0.01),arrested cells in G_2/M phase and induced apoptosis by activating caspase-3,PARP and Bax.SCRE treatment also correlated with inhibition of NF- κB and downregulation of Bcl-2,Bcl-XL,cIAP-1,XIAP and AKT.SCRE can promote chemosensitivity to cisplatin and adriamycin in A2780 cells(P<0.01).Conclusion:SCR effectively inhibits NF- κB,induces apoptosis and reduces chemoresistance to cisplatin and adriamycin in ovarian cancer cells,which might be its molecular basis for treating ovarian cancer.
Objective: To study the antitumor effects and associated mechanisms of extract of the Smilax china L rhizome (SCR) on ovarian cancer cells. Methods: Ovarian cancer cells A2780 were treated with different concentrations of SCR extract (SCRE), and compared with controls. Effects on cell growth were evaluated by cell counting kit-8 (CCK-8) assay; proliferation effects by EdU incorporation assay; cell cycle by propidium iodide staining; apoptosis by annexin V-fluorescein isothiocyanate / propidium iodide; cellular distribution of nuclear factor- k B (NF-κB) by immunofluorescence; protein levels of NF-κB, caspase-3, poly-adenosine diphosphate (ADP) -ribose polymerase (PARP) (XIAP), B-cell lymphoma-extra large (Bcl-XL), B-cell lymphoma-2 of SCRE combined with cisplatin or adriamycin on A2780 cells by CCK-8 assay. Results: SCRE suppressed A2780 cell prol arrested cells in G2 / M phase and induced apoptosis by activating caspase-3, PARP and Bax. SCRE treatment also correlated with inhibition of NF- [kappa] B and downregulation of Bcl-2, Bcl-XL, cIAP-1, XIAP and AKT.SCRE can promote chemosensitivity to cisplatin and adriamycin in A2780 cells (P <0.01) .Conclusion: SCR efficiently inhibits NF- κB, induces apoptosis and reduces chemoresistance to cisplatin and adriamycin in ovarian cancer cells, which might be its molecular basis for treating ovarian cancer.