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目的：研究抑癌基因 Ｐ５３ 及其表达产物 Ｐ５３ 蛋白在肾上腺嗜铬细胞瘤中的表达，比较 Ｐ５３ ｍ Ｒ Ｎ Ａ 表达与 Ｐ５３ 蛋白表达的关系。方法：应用免疫组织化学及核酸分子原位杂交方法检测 Ｐ５３ 基因及其产物的表达情况。结果：原位杂交显示， Ｐ５３ ｍ Ｒ Ｎ Ａ 在肾上腺嗜铬细胞瘤中表达为２４．０％ （７／２９），在恶性肾上腺嗜铬细胞瘤中为１４．０％ （１／７）；免疫组织化学显示， Ｐ５３ 蛋白在肾上腺嗜铬细胞癌中表达为７９．３％ （２３／２９），在恶性肾上腺嗜铬细胞瘤中为７１．０％ （５／７）。结论：在肾上腺嗜铬细胞瘤中，存在 Ｐ５３ 基因突变，推测 Ｐ５３ 基因与肾上腺嗜铬细胞瘤发生、发展有显著相关性。
Objective: To investigate the expression of tumor suppressor gene P53 and its expression product P53 in adrenal pheochromocytoma and to compare the relationship between P53 m R N A expression and P53 protein expression. Methods: The expression of P53 gene and its products were detected by immunohistochemistry and nucleic acid in situ hybridization. RESULTS: In situ hybridization showed that P53 m R N A was expressed in 24.0% (7/29) of adrenal pheochromocytoma and 14.0% (1/7) in malignant adrenal pheochromocytoma. Immunohistochemistry showed that P53 protein was expressed in 79.3% (23/29) of adrenal pheochromocytoma and 71.0% (5/7) in malignant adrenal pheochromocytoma. Conclusion: There is a mutation in P53 gene in adrenal pheochromocytoma. It is speculated that P53 gene is significantly associated with the occurrence and development of adrenal pheochromocytoma.