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目的研究乙型肝炎病毒(HBV)基因型与肝脏损伤程度及病毒载量(HBV-DNA)的相关性。方法用微板核酸杂交-ELISA方法对108例慢性HBV感染者进行HBV基因分型,PCR 荧光定量检测血清HBV-DNA水平。结果基因型B型29例(26．9％),C型51例(47．2％),混合型(C+D／B+C)16例(14．8％),D型4例(3．7％),未分型8例(7．4％)。C型发展致慢性乙型肝炎 (CHB)、肝硬化(LC)和肝细胞癌(HCC)比例显著高于B型,分别为49．0％比41．4％(x2=3．91,P< 0．05);17．6％比6．9％(x2=4．90,P<0．05);13．7％比3．4％(x2=5．06,P<0．05)。C型和混合型 HBV-DNA水平高于B型(1．14×107比2,20×107比1．60×106拷贝／ml,P<0．05)。结论 C型 HBV感染较易引起严重肝脏损伤,同时,HBV基因型可能是影响HBV复制的重要因素之一。
Objective To study the relationship between hepatitis B virus (HBV) genotype and degree of liver damage and viral load (HBV-DNA). Methods HBV genotyping was performed in 108 patients with chronic HBV infection by ELISA and microplate nucleic acid hybridization. The level of serum HBV DNA was detected by PCR. Results Genotype B was found in 29 cases (26.9%), 51 cases (47.2%) in C type, 16 cases (14.8%) in mixed type (C + D / B + C) 3.7%), 8 cases (7.4%) were not typed. The proportion of type C chronic hepatitis B (CHB), cirrhosis (LC) and hepatocellular carcinoma (HCC) was significantly higher than that of type B (49.0% vs. 41.4%, respectively <0.05), 17.6% vs 6.9% (x2 = 4.90, P <0.05), 13.7% vs 3.4% (x2 = 5.06, P <0.05) ). Type C and mixed HBV-DNA levels were higher than those of type B (1.14 × 107 vs. 2, 20 × 107 vs. 1.60 × 106 copies / ml, P <0.05). Conclusions Type C HBV infection is more likely to cause severe liver injury. At the same time, HBV genotype may be one of the important factors that affect HBV replication.