论文部分内容阅读
用直接杀伤,细胞生长的抑制及克隆形成能力的抑制等方法,试验了亚甲兰体外的抗肿瘤效应,发现其抗瘤作用有剂量及时间依赖性两种特征1μg/ml亚甲兰处理4小时,从肺腺癌细胞LTEP—a_3克隆存活为对照的10~(-1),48小时为10~(-2)72小时为10~(-3),亚甲兰浓度在1μg/ml以以上,延长作用时间显著增强了其对癌细胞的杀伤力,当处理时间在24小时或为上时,杀伤效应与浓度相关,亚甲兰对a_3癌细胞主要损伤部位为线粒体。
The anti-tumor effect of methylene blue was tested in vitro with direct killing, inhibition of cell growth, and inhibition of colony formation ability. It was found that the anti-tumor effect was dose- and time-dependent. Features 1 μg/ml methylene blue treatment 4 Hours, cloned from the lung adenocarcinoma cell LTEP-a_3 survived as the control 10~(-1), 48 hours to 10~(-2) 72 hours to 10~(-3), and methylene blue concentration to 1 μg/ml. Above, the prolonged action time significantly enhanced its lethality to cancer cells. When the treatment time was 24 hours or above, the killing effect was related to the concentration, and methylene blue was the major site of damage to the a_3 cancer cells as mitochondria.