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目的探索QHF复方对肝癌细胞增殖的抑制活性。方法选取人肝癌细胞株SMMC7721为受试对象,分别予以0(正常对照组)、QHF1、QHF2、QHF3、QHF4、QHF5、2.5μg/ml顺铂后培养24h、48h和72h,检测细胞光密度,计算增殖抑制率。以0(正常对照组)、QHF1、QHF2、QHF3、QHF4、QHF5、2.5μg/ml顺铂(阳性对照组)干预细胞72h后,检测细胞周期分布、凋亡率和Bax、Bcl-2基因表达水平。结果与正常对照组相比,QHF复方可显著抑制SMMC7721细胞的增殖,并呈现时间依赖性、剂量依赖性规律;该复方可显著增加受试细胞株G0/G1期、G2/M期细胞分布比例,阻止细胞DNA复制而促进凋亡;RT-PCR结果显示QHF复方可显著上调促凋亡蛋白Bax基因,而显著下调抑制凋亡蛋白Bcl-2基因。结论QHF复方可通过调控细胞周期分布和凋亡信号通路关键基因表达水平而实现对肝癌细胞增殖的显著抑制。
Objective To explore the inhibitory activity of QHF compound on hepatocellular carcinoma cell proliferation. Methods Human hepatocellular carcinoma cell line SMMC7721 was selected as experimental subjects and cultured for 24 hours, 48 hours and 72 hours after treatment with 0 (normal control group), QHF1, QHF2, QHF3, QHF4, QHF5 and 2.5μg / ml cisplatin respectively. Calculate the proliferation inhibition rate. The cell cycle distribution, the apoptosis rate and the expression of Bcl-2 and Bcl-2 genes were detected after treated with 0 (normal control), QHF1, QHF2, QHF3, QHF4, QHF5 and 2.5μg / ml cisplatin (positive control) Level. Results Compared with the normal control group, QHF compound could significantly inhibit the proliferation of SMMC7721 cells in a time-dependent and dose-dependent manner. The compound could significantly increase the proportion of cells in G0 / G1 and G2 / M phases , Preventing DNA replication and promoting apoptosis; RT-PCR results showed that QHF compound could significantly up-regulate the Bax gene of pro-apoptotic protein and significantly down-regulate the apoptosis-inhibiting protein Bcl-2 gene. Conclusion QHF compound can significantly inhibit the proliferation of hepatoma cells by regulating the cell cycle distribution and the expression level of key genes in apoptosis signaling pathway.