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目的:建立测定七归滴丸中阿魏酸、三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量测定方法。方法:采用C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.6%冰醋酸(30∶70),检测波长为323 nm,测定阿魏酸;采用C18柱(4.6 mm×250 mm,5μm),乙腈-水梯度洗脱,流速0.8 mL·min-1,检测波长203 nm,测定三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1。结果:阿魏酸的回收率为99.18%(RSD 1.4%);三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的回收率分别为98.77%(RSD1.71%),98.94%(RSD 1.58%),99.48%(RSD 1.65%)。结论:此法准确、可靠,重复性好,可用于控制七归滴丸的质量。
Objective: To establish a method for the determination of ferulic acid, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Qiugui dripping pills. Methods: Ferulic acid was determined by C18 column (4.6 mm × 250 mm, 5 μm) using methanol-0.6% glacial acetic acid (30:70) at a detection wavelength of 323 nm. , 5μm), acetonitrile-water gradient, the flow rate of 0.8 mL · min-1, detection wavelength of 203 nm, notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1. Results: The recovery rate of ferulic acid was 99.18% (RSD 1.4%). The recoveries of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 98.77% (RSD 1.71%) and 98.94% (RSD 1.58%) respectively, , 99.48% (RSD 1.65%). Conclusion: This method is accurate, reliable, reproducible, and can be used to control the quality of Qidan dropping pills.