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探讨原发性肾病综合征(PNS)患儿血、尿及肾组织中白细胞介素8(IL-8)的变化及其临床意义。采用ELISA双抗体夹心法检测PNS患儿血清及尿IL-8水平,用免疫组化ABC法检测PNS患儿肾组织中IL-8的临床分布。结果:病理上为增生性的PNS患儿肾病极期血清及尿IL-8水平明显高于肾病缓解期及对照组,尿IL-8与24小时尿蛋白定量、肾小球系膜增生程度呈正相关,相应的肾组织中抗IL-8单抗染色阳性。病理上为非增生性的PNS患儿肾病极期血清IL-8水平也明显高于肾病缓解期及对照组,而尿IL-8在肾病极期及缓解期均无异常,相应的肾组织中抗IL-8单抗染色阴性。检测尿及血清IL-8可作为小儿肾病活动及临床分型的参考指标。
To investigate the changes of interleukin-8 (IL-8) in blood, urine and kidney of children with primary nephrotic syndrome (PNS) and its clinical significance. Serum and urinary IL-8 levels were measured by sandwich enzyme-linked immunosorbent assay (ELISA) and the distribution of IL-8 in renal tissue of children with PNS was detected by immunohistochemical ABC method. Results: The pathological hyperplastic PNS children with stage serum nephropathy and urine IL-8 levels were significantly higher than the nephropathy remission and control group, urinary IL-8 and 24-hour urinary protein, glomerular mesangial proliferation were positive Related, the corresponding renal tissue anti-IL-8 monoclonal staining positive. Pathologically, non-proliferative PNS children with stage serum nephrotic serum IL-8 levels were significantly higher than the nephropathy remission and control group, while urinary IL-8 in the nephrotic stage and remission were normal, the corresponding renal tissue Anti-IL-8 monoclonal antibody staining negative. Detection of urine and serum IL-8 can be used as pediatric nephropathy activity and clinical classification of the reference index.