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目的:研究木犀草素在4-(2-吡啶偶氮)间苯二酚(PAR)导电聚合膜修饰玻碳电极上的电化学行为,建立测定木犀草素含量的电化学分析新方法。方法:采用循环伏安法研究木犀草素在导电聚合膜修饰玻碳电极上的电化学行为及其电极反应机理,以差示脉冲伏安法建立了检测木犀草素含量的电化学分析新方法。结果:在磷酸盐缓冲液(pH 4.0)中,在-0.2～+0.8 V范围内木犀草素在PAR膜修饰电极表面是受吸附控制,发生准可逆单电子转移电极反应过程,电子转移系数α=0.47;在导电聚合膜厚度为40圈(100mV.s-1),富集电位-0.2 V,富集时间180 s,利用差示脉冲伏安法可测得其氧化峰电流Ip与浓度分别在3.0×10-8～1.0×10-6mol·L-1内呈良好的线性关系,检出限为1.0×10-8mol·L-1。结论:本法操作简单、快速、灵敏、准确,并可为木犀草素的质量控制提供科学依据。
OBJECTIVE: To study the electrochemical behavior of luteolin on the glassy carbon electrode modified by 4- (2-pyridylazo) resorcinol (PAR) conductive polymer and establish a new electrochemical method for the determination of luteolin. Methods: The cyclic voltammetry was used to study the electrochemical behavior of luteolin at the conductive polymer film modified glassy carbon electrode and its electrode reaction mechanism. A new electrochemical method was developed for the determination of luteolin by differential pulse voltammetry . Results: In the range of -0.2 ~ + 0.8 V, Luteolin was adsorbed and controlled on the surface of PAR modified electrode in phosphate buffer solution (pH 4.0), and the quasi-reversible one-electron transfer electrode reaction occurred. The electron transfer coefficient α = 0.47, respectively. When the thickness of the conductive polymer film is 40 cycles (100mV.s-1), the enrichment potential is -0.2 V and the enrichment time is 180 s, the peak current Ip and the concentration of the oxide peak can be measured by differential pulse voltammetry The linear range was 3.0 × 10-8 ~ 1.0 × 10-6 mol·L-1 with the detection limit of 1.0 × 10-8 mol·L-1. Conclusion: The method is simple, rapid, sensitive and accurate, and can provide a scientific basis for the quality control of luteolin.