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目的分析菏泽市艾滋病确证实验室人类免疫缺陷病毒(HIV)抗体检测结果,为指导艾滋病防治提供科学依据。方法对初筛阳性标本,采用酶联免疫吸附试验和胶体硒法进行复检,2种试剂均呈阳性或一阴一阳的用蛋白免疫印迹法(WB)进行确证试验,确证为阳性病例进行流行病学分析。结果初筛样本236份阳性,复检136份阳性,WB确证HIV-1抗体阳性125份,抗体不确定8份,阴性3份。14个人群检出HIV-1抗体阳性,其中以自愿检测咨询构成比最高,为51.2%(64/125)。感染者文化水平较低,以初中及以下为主,占76.0%(95/125)。以20岁~45岁的青壮年为主,占76.0%(95/125)。传播途径以男男同性传播为主,占52.0%(65/125)。结论建议进一步加大HIV筛查力度,尤其加强男男性行为的人群管理,提高该人群的检测率,加强艾滋病健康教育和干预的力度,遏制艾滋病的蔓延。
Objective To analyze the results of HIV antibody test in AIDS-confirmed laboratory in Heze City and provide a scientific basis for AIDS prevention and treatment. Methods Positive samples were screened by enzyme-linked immunosorbent assay (ELISA) and selenium-selenium method. The positive results were confirmed by Western blotting (WB) Epidemiological analysis. Results A total of 236 positive samples were screened and 136 samples were positive. WB positive HIV-1 antibody positive 125 copies, antibody undetermined 8 copies, negative 3 copies. HIV-1 antibody was detected in 14 cohorts, of which 51.2% (64/125) had the highest ratio of voluntary testing counseling. The level of HIV infection was low, mainly in middle school and below, accounting for 76.0% (95/125). The majority of young adults aged 20 to 45 accounted for 76.0% (95/125). The main route of transmission was MSM, accounting for 52.0% (65/125). Conclusions It is suggested to further increase HIV screening, especially to strengthen the management of men who have sex with men, to improve the detection rate of the population, to strengthen AIDS health education and intervention, and to curb the spread of AIDS.