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目的 探讨微生物对青蒿素 ( )的转化作用。方法 通过华根霉和雅致小克银汉霉在土豆培养基中发酵产酶 ,对青蒿素进行转化。结果 两株菌对底物均有转化作用 ,分离出去氧青蒿素 deoxyartem isinin ( ) ,3α-羟基去氧青蒿素 3α- hydroxydeoxyartemisinin( )和 9β-羟基青蒿素 9β- hydroxyartemisinin( )共 3个产物 ,其中 为一新化合物 ,同时振荡条件下底物在无菌培养基中也能发生微量转化得到产物 。结论 青蒿素易被实验两株真菌转化 ,同时过氧桥也易断裂而失去一个氧原子成为去氧青蒿素 ,丧失抗疟活性 ,起作用的可能是土豆培养基中的铁元素
Objective To investigate the transformation of artemisinin () by microorganisms. Methods The enzyme was fermented by Rhizopus nucifera and Nicotiana oleifera in potato culture medium to transform artemisinin. RESULTS: Both strains transformed the substrate and isolated oxyartemisinin (deoxyartem isinin), 3α-hydroxydeoxyartemisinin (3α-hydroxydeoxyartemisinin) and 9β-hydroxyartemisinin (9β-hydroxyartemisinin). One product, which is a new compound, is also capable of undergoing microconversion in the sterile medium under the shaking conditions to obtain the product. Conclusion Artemisinin is easily transformed by the two fungi, and the peroxy bridge is also easily broken and loses an oxygen atom to become deoxyartemisinin. It loses antimalarial activity. It may be iron in the potato culture medium.