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1962年我们在师卫生科的条件下,开展了钩端螺旋体病的病原体(以下简称钩端)分离培养工作,不断总结经验,改进工作方法,到目前为止已能顺利地进行分离培养,现就点滴经验介绍如下:钩端分离培养经过1.培养基:采用柯尔索夫氏(以下简称柯氏)液体培养基效果较好,由于需要家兔血清,但市售家兔多数含有自然抗体影响钩端生长,就以自繁自养解决,两年来繁殖了家兔40余头,保证了制作培养基所需的血清。
In 1962, under the conditions of teacher division, we carried out the isolation and culture of the pathogen of leptospirosis (hereinafter referred to as the hook end), constantly sum up experience and improve the working methods, so far we have been able to successfully carry out isolation and culture, now Experience is introduced as follows: Hook end separated culture after 1. Medium: Korstsov (hereinafter referred to as Kirschner) liquid medium better, due to the need for rabbit serum, but the majority of commercial rabbits contain natural antibodies Crocodile growth, to self-autotrophic solution, breeding two rabbits more than 40 head, ensuring the production of serum required for the medium.