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在高海拔地区的黄壤以田间试验与盆栽试验，用双标记￣（１５）ＮＨ＿４￣（１５）ＮＯ＿３进行研究。结果表明，烤烟氮素积累量表现为中位叶＞上位叶＞下位叶；烟叶的蛋白质、烟碱含量则是上位叶＞中位叶＞下位叶，它们的含量与肥料短进入的比例呈曲线负相关；肥料进入的比例呈下位叶＞中位叶＞上位叶的相反趋势。本文最后提出贵州不同烤烟产区施氮量应控制在４．５～７．５ｋｇ／亩之间，因４～６月份是雨季，氮肥应多次分施为宜，以免遭受淋失
Field experiments and pot experiments were conducted on yellow soil at high altitude, and double labeling of ~ (15) NH_4 ~ (15) NO_3 was carried out. The results showed that the accumulation of nitrogen in flue-cured tobacco showed the middle leaf> upper leaf> lower leaf. The content of protein and nicotine in tobacco leaf was higher leaf> middle leaf> lower leaf. The ratio of their content to the short- Negative correlation; the proportion of fertilizer entering the lower lobe> middle lobe> upper lobe opposite trend. In the end, the paper puts forward that the nitrogen application rate in different flue-cured tobacco producing areas in Guizhou should be controlled between 4.5 ~ 7.5kg / mu, because the rainy season is from April to June, and nitrogenous fertilizer should be applied multiple times in order to avoid leaching