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大鼠15只,先将动物视神经切断,再移植坐骨神经段(PN、长约2mm/每段)到眼玻璃体内,动物分别存活2、4、8周后,取视网膜,沿视轴纵切,Nissl染色,显微镜观察,并用计算机图像分析仪(IBAS-2000)测量部分视网膜节细胞胞体截面积。结果证明,在PN的诱导下,存活2、4、8周时的视网膜节细胞层均出现巨大视神经细胞,胞体呈椭圆形,其胞体平均截面积分别为75.3±16.0μm~2、93.7±33.8μm~2和81.2±19.6μm~2;其中特巨细胞构成比分别为7.6％、34.6％和17.5％;二者均以存活4周时为最大,三组呈现一个由小到大再减小的变化趋势。经统计学检验,三组之间均有显著性差异(P<0.01)。
Fifteen rats were sacrificed and the optic nerve was excised. The sciatic nerve segment (PN, length about 2 mm / segment) was transplanted into the vitreous of the eye. The animals survived for 2, 4 and 8 weeks, respectively. The retina was transected along the optic axis, Nissl staining, microscopic observation, and part of the retinal ganglion cell body cell body cross-sectional area was measured using a computer image analyzer (IBAS-2000). The results showed that under the induction of PN, giant retinal ganglion cells appeared in the retinal ganglion cells at 2, 4 and 8 weeks of survival, with oval cell bodies with average cell cross sections of 75.3 ± 16.0μm ~ 2,93.7 ± 33.8 μm 2 and 81.2 ± 19.6μm 2, respectively. The proportions of extra giant cells were 7.6%, 34.6% and 17.5% respectively. Both of them were the largest at 4 weeks of survival, The trend of change. Statistically, there was a significant difference between the three groups (P <0.01).