传统的线虫形态学鉴定存在经验性强和幼虫难以鉴定等不足。本文根据线虫ITS基因序列多态性 ,设计鳞球茎茎线虫 (Ditylenchusdipsaci)特异的TaqMan探针 ,建立了实时荧光PCR检测方法。对茎线虫属的鉴定实验表明 :只有鳞球茎茎线虫产生特异性荧光信号 ,同属的其他线虫均检测到荧光信号。整个检测过程只需 30min到 2h ,能够满足口岸检验检疫快速通关的要求。 Traditional nematode morphological identification of experiencing strong and larvae difficult to identify such shortcomings. In this paper, we designed a TaqMan probe specific for Ditylenchus dipsaci according to the ITS sequence of nematode, and established a real-time fluorescence PCR detection method. The identification of Stem nematode experiments showed that: only D. dipsaci produce specific fluorescence signal, the other nematodes are detected fluorescence signal. The entire testing process only 30min to 2h, to meet the rapid inspection and quarantine requirements of the port.