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利用从主胰管注射自身胆汁1ml/kg体重复制的狗急性出血坏死性胰腺炎模型,观察预防应用磷脂酶A_2抑制剂氯喹对肺水肿的影响。实验结果表明胰腺炎组肺系数和肺湿/干重比显著增高,肺血液量和肺血管外水量显著增多,同时支气管肺泡灌洗液中的总蛋白浓度和细胞计数也显著增高。细胞分类可见胰腺炎组巨噬细胞比例显著下降,多形核白细胞和肺泡Ⅱ型上皮细胞比例显著增加。预防应用氯喹对急性胰腺炎肺水肿有显著改善作用,细胞分类中多形核白细胞比例也下降。提示磷脂酶A_2在急性胰腺炎时肺水肿的发生中起重要作用,氯喹对肺水肿的治疗有临床应用价值。
The model of acute hemorrhagic necrotizing pancreatitis in rabbits injected with 1ml / kg body weight of bile from the main pancreatic duct was used to observe the preventive effect of chloroquine, a phospholipase A2 inhibitor, on pulmonary edema. The experimental results showed that the lung coefficient and lung wet / dry weight ratio of pancreatitis group were significantly increased, the pulmonary blood volume and pulmonary extravascular water volume increased significantly, meanwhile, the total protein concentration and cell count in bronchoalveolar lavage fluid were also significantly increased. The cell classification showed that the proportion of macrophages in pancreatitis group was significantly decreased, the ratio of polymorphonuclear leukocytes and alveolar type Ⅱ epithelial cells increased significantly. Chloroquine prevention and treatment of acute pancreatitis pulmonary edema significantly improved the role of cell sorting in the proportion of polymorphonuclear leukocytes also declined. It is suggested that phospholipase A_2 plays an important role in the development of pulmonary edema in acute pancreatitis. Chloroquine has clinical value in the treatment of pulmonary edema.