红厚壳(Calophyllum inophyllum)为藤黄科红厚壳属多年生木本植物,有很高的药用价值。该研究以红厚壳带节茎段为外植体,探讨生长调节剂对腋芽萌发及丛生芽诱导、伸长和试管苗生根的影响。研究结果表明,外植体腋芽萌发和丛生芽诱导效果最好的培养基是MS+NAA1.0+TDZ0.5,在此条件下培养21天后,转入添加0.5 g·L–1活性炭且无生长调节剂的MS培养基,可有效促进不定芽的伸长。将带不定芽的外植体先在附加1.0 mg·L–1NAA的1/2MS培养基上进行生根诱导4周,之后转入附加1.0 g·L–1活性炭的无激素培养基进行根的伸长培养,这样的两步生根法能有效促进红厚壳生根。 Calophyllum inophyllum is a perennial woody plant belonging to the genus Garcinia cambogia, which has high medicinal value. In this study, the stem segments of Rhizomucor pachyrhizi were used as explants to investigate the effects of growth regulators on the induction of axillary buds, the induction of buds, the elongation and the rooting of test-tube seedlings. The results showed that the best medium for inducing axillary buds and clustered shoots was MS + NAA1.0 + TDZ0.5. After cultured for 21 days under these conditions, 0.5g · L-1 activated carbon MS medium for growth regulator can effectively promote the elongation of adventitious buds. The explants with adventitious buds were first rooted on 1/2 MS medium supplemented with 1.0 mg · L -1 NAA for 4 weeks and then transferred to a hormone-free medium supplemented with 1.0 g · L -1 activated carbon for root elongation Long training, such a two-step rooting method can effectively promote the red thick shell rooting.