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为了在受体水平探讨神经与内分泌的相互关系,本文采用免疫细胞化学双重反应方法研究了雌性大鼠雌激素受体和代谢型谷氨酸受体1 亚型在前脑的分布与共存。用小鼠抗雌激素受体血清和兔抗代谢型谷氨酸受体1 亚型血清分别孵育或共同孵育切片,ABC 法显示前者的反应产物呈粉红色(或棕色),后者呈黑色。结果表明,两者在大脑皮质和下丘脑都有较广泛的分布,后者除神经细胞外,还分布于侧脑室的室管膜细胞。在双重免疫细胞化学反应的条件下可见3 种细胞:雌激素受体阳性细胞,代谢型谷氨酸受体1 亚型阳性细胞和双重反应阳性细胞。双重反应阳性细胞所占的比例因部位而不同。以上结果提示:雌激素和谷氨酸可通过共存于同一神经细胞上的相应受体,在信使、基因和转录水平相互作用,调节神经细胞的功能状态
In order to explore the relationship between neuroendocrine in the receptor level, we used immunocytochemical double reaction method to study the distribution and coexistence of estrogen receptor and metabotropic glutamate receptor subtype 1 in the forebrain. Slides were incubated with mouse anti-estrogen receptor serum and rabbit anti-metabotropic glutamate receptor subtype 1 sera, respectively. The ABC method showed that the former reaction product was pink (or brown) and the latter was black. The results show that the two have a more extensive distribution in the cerebral cortex and hypothalamus, the latter in addition to nerve cells, but also distributed in the lateral ventricle ependymal cells. Under the conditions of dual immunocytochemistry, three kinds of cells were found: estrogen receptor positive cells, metabotropic glutamate receptor subtype 1 positive cells and double reaction positive cells. The proportion of double-reactive cells varies by site. These results suggest that estrogen and glutamate can interact with receptors on the same nerve cells via messenger, gene and transcription to regulate the functional status of nerve cells