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本文探讨了HBD1对脐血CD4~+T细胞的增殖、细胞表面分子CD69、CD25、CD40L和CD45RO的表达及B细胞CD80、CD86分子表达的影响,并以成人外周血CD4~+T细胞作对照。采用CCK-8增殖实验检测CD4~+T细胞的增殖情况,采用流式细胞术检测CD80、CD86、CD69、CD25、CD45RO和CD40L的表达情况。结果表明:HBD1促进了脐血CD4~+T细胞的增殖,CD69、CD25、CD40L表达上调;B细胞CD80、CD86的表达也上调,但并未促进CD45RO表达的上调。而相同处理条件下,HBD1对成人外周血CD4~+T细胞作用不明显,提示HBD1对脐血来源CD4~+T细胞具有一定的免疫调节作用。
In this paper, the effects of HBD1 on the proliferation of CD4 ~ + T cells, the expression of CD69, CD25, CD40L and CD45RO on cord blood and the expression of CD80 and CD86 molecules on cord blood were investigated. CD4 ~ + T cells in peripheral blood were used as controls . The proliferation of CD4 ~ + T cells was detected by CCK-8 proliferation assay. The expressions of CD80, CD86, CD69, CD25, CD45RO and CD40L were detected by flow cytometry. The results showed that: HBD1 promoted the proliferation of CD4 ~ + T cells in umbilical cord blood, the expression of CD69, CD25 and CD40L were up-regulated; the expressions of CD80 and CD86 were also up-regulated in B cells but did not promote the up-regulation of CD45RO expression. However, under the same treatment conditions, HBD1 had no obvious effect on CD4 ~ + T cells in adult peripheral blood, suggesting that HBD1 has some immunoregulatory effects on CD4 ~ + T cells derived from cord blood.