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目的:预测并分析比较衣原体MOMP蛋白的二级结构和B细胞表位。方法:以各株衣原体MOMP蛋白的氨基酸序列为基础,采用Gamier-Robson法、Chou-Fasman法和Karplus-Schulz法预测蛋白二级结构;按Kyte-Doolittle法、Emini法和Jame-son-Wolf法预测蛋白的抗原表位;以ClustalX软件序列比对分析其多变区。结果:各株MOMP蛋白含多个抗原位点,各序列在第80-107、165-178、249-264、332-346位序列高度差异,并出现比对“空沟”。多个强表位位于序列保守区内。结论:MOMP蛋白有较强的免疫原性,预测的抗原位点为之后蛋白相互作用研究、单抗制备、亚单位疫苗设计提供了理论依据。
OBJECTIVE: To predict and analyze the secondary structure and B cell epitopes of Chlamydia MOMP protein. Methods: Based on the amino acid sequence of MOMP in each strain of Chlamydia, Gamier-Robson method, Chou-Fasman method and Karplus-Schulz method were used to predict the protein secondary structure. According to Kyte-Doolittle method, Emini method and Jameson-Wolf method Predict the antigenic epitope of the protein; analyze its variable region by alignment with ClustalX software. Results: The MOMP protein of each strain contained multiple antigenic sites. The sequence of each MOMP protein was highly divergent at the 80th to 107th, 165th to 178th, 249th to 264th, and324th to 346th positions. Multiple strong epitopes are located in the conserved region of the sequence. CONCLUSION: MOMP protein has strong immunogenicity. The predicted antigenic site provides the theoretical basis for protein interaction research, preparation of monoclonal antibody and subunit vaccine design.