目的　观察神经丝的完整性是否对线状溶酶体的形状与分布有影响。　方法　采用SABC单克隆抗体免疫组织化学法、免疫电镜及酶组织化学、电镜酶组织化学技术观察钒酸盐处理后培养的神经元内神经丝蛋白NF H及线状溶酶体的改变。　结果　当神经丝的完整性被破坏时 ,神经丝蛋白向核周聚集 ,并伴随线状溶酶体亦向核周聚集并形状改变。　结论　钒酸盐通过多途径作用 ,使神经丝过度磷酸化 ,失去装配能力 ,当神经丝结构被破坏时 ,线状溶酶体的形状及分布都发生变化。线状溶酶体的形状与分布对神经丝的完整性有依赖作用。 Objective To observe whether the integrity of neurofilament affects the shape and distribution of lysosomes. Methods SABC monoclonal antibody immunohistochemistry, immunoelectron microscopy and enzyme histochemistry, electron microscopy were used to observe the changes of neurofilament NF H and mitochondrial lysosomes in neurons cultured with vanadate. Results When the integrity of the neurofilament was disrupted, the neurofilament aggregated toward the perinuclear area, with linear lysosomes also aggregating and changing shape toward the perinuclear area. CONCLUSION Vanadate acts through multiple pathways that phosphorylate neurofilaments and lose their assembly ability. When the structure of the nerve filaments is destroyed, the shape and distribution of the lysosomes are changed. The shape and distribution of mitochondria are dependent on the integrity of neurofilaments.