玉米干旱胁迫相关突变体在发掘玉米耐旱关键基因研究中具有重要利用价值。在玉米自交系综31的田间扩繁过程中,发现一个玉米干旱胁迫敏感的自然突变体,该突变体在轻度干旱条件下叶片发生卷曲,严重干旱时叶尖变黄,衰老坏死。遗传分析表明突变性状受1对主效单基因控制,表现为隐性遗传,将突变基因命名为DS。利用B73与突变体ds组配F2分离群体,以干旱条件下叶片是否卷曲为指标,将DS基因初定位在第3号染色体SSR标记umc1772和umc2158之间,物理距离为5 Mb。以上研究结果为该基因的克隆及功能分析奠定了基础。 Mutants related to drought stress in maize have important value in the study of key genes of drought resistance in maize. In the process of field propagation of maize inbreds 31, a natural mutant sensitive to drought stress was found in the maize plants. The mutants were curled under mild drought conditions. The leaves became yellow and senescent in severe drought. Genetic analysis showed that the mutant trait was controlled by a single dominant single gene and showed recessive inheritance. The mutant gene was named DS. The F2 segregation population of B73 and mutant ds was used to determine whether DS was initially located on the chromosome 3 SSR markers umc1772 and umc2158 under the condition of drought or not. The physical distance was 5 Mb. The above results provide a basis for the cloning and functional analysis of this gene.