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研究了固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应 ,系统探讨了振荡速度、底物浓度、固定化细胞浓度、pH值和反应温度对反应速度、产率和产物光学纯度的影响。结果表明 ,上述因素对固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应均有较显著的影响。振荡速度以 1 5 0r min为宜 ,底物浓度和固定化细胞浓度分别为 1 4mmol L和 0 1 5g mL较佳 ,适宜的pH值为 7 3 ,最佳反应温度为 2 5℃～ 3 0℃。在该优化反应条件下 ,反应最大产率和产物的光学纯度分别高达 84 9%和 90 2 %ee。
The asymmetric reduction of trimethylsilyl chloride catalyzed by immobilized Saccharomyces cerevisiae cells was studied. The effects of shaking speed, substrate concentration, immobilized cell concentration, pH value and reaction temperature on the reaction rate, yield and optical purity influences. The results showed that the above factors had a significant effect on the asymmetric reduction of trimethylsilyl ketone catalyzed by immobilized Saccharomyces cerevisiae cells. The optimal shaking speed was 150 min, substrate concentration and immobilized cell concentration were 14 mmol L and 0 15 g mL, respectively. The optimum pH value was 7 3 and the optimal reaction temperature was 25 ℃ ~ 30 ℃. Under this optimized reaction conditions, the maximum yield of the reaction and the optical purity of the product were 84 9% and 90 2% ee, respectively.