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利用邻苯二甲醛（ＯＰＡ）柱前衍生－反相高效液相色谱（ＨＰＬＣ）分离－荧光检测的分析方法测定了海马区细胞外液中游离氨基酸的含量， 对少至５ μＬ的微透析液中的１８ 种氨基酸进行了定性和定量分析． 当信噪比为３ 时， 各氨基酸的最小检出量在０．５～６．０ ｐｍ ｏｌ之间． 实验结果表明， 操作式条件反射学习训练和与之形成的巩固记忆可使海马区递质氨基酸及其它游离氨基酸的释放和／或代谢量增强． 这一结果支持海马是与学习记忆有密切关系的脑神经核团的看法．
The contents of free amino acids in the extracellular fluid of hippocampus were measured by OPA column derivatization-reversed-phase high performance liquid chromatography (HPLC)-fluorescence detection. The effect of as little as 5 μL of microdialysis solution The 18 kinds of amino acids were qualitative and quantitative analysis. When the signal to noise ratio is 3, the minimum detectable amount of each amino acid is between 0.5 and 6.0 pmol. The experimental results show that the manipulative conditioned reflex learning training and the consolidation memory formed therewith can enhance the release and / or metabolism of transmitter amino acids and other free amino acids in the hippocampus. This result supports the idea that the hippocampus is a brain nucleus closely related to learning and memory.