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目的验证PuriTyperTM纯化试剂盒各项性能指标和法医学应用价值。方法收集及制备抗凝血液、常见案件检材(唾液、烟头、精液、毛发、指甲、骨骼及组织块)、斑痕样本(血斑、唾液斑、精斑)以及模拟添加抑制剂和模仿自然环境中放置的血斑。采用PuriTyperTM纯化试剂盒提取纯化并进行DNA定量,IdentifilerTM复合扩增试剂盒扩增,产物经ABI 3130遗传分析仪进行检测,Genemapper软件分析结果,对该试剂盒灵敏度、稳定性、重复性、检材适应性进行测试。结果采用该试剂盒提取0.1～40μL血液分别获得0.042～26.45ng/μL的DNA。3种斑痕样本DNA产量平行试验结果稳定。不同类型检材重复检验所获IPC的CT平均值在27.60至28.03之间。常见案件检材所得分型与已知结果均一致。结论 PuriTyperTM纯化试剂盒能够满足法医DNA检验的要求,对法医学实践具有重要的应用价值。
Objective To verify the performance of PuriTyperTM purification kit and forensic application value. Methods Anticoagulant blood samples were collected and collected. Samples of common cases (saliva, cigarette butts, semen, hair, nails, bones and tissue blocks), scar samples (blood spots, saliva plaques and seminal spots) were collected and simulated to simulate the addition of inhibitors and mimics of the natural environment Placed in the blood spots. PuriTyperTM purification kit was used to purify and quantify DNA. The amplified product was amplified by IdentifilerTM multiplex amplification kit. The product was detected by ABI 3130 genetic analyzer. Genemapper software was used to analyze the sensitivity, stability, repeatability, Adaptability test. Results Using this kit, 0.1 ~ 40μL blood was extracted to obtain 0.042 ~ 26.45ng / μL DNA respectively. The parallel results of DNA yield of three kinds of scar samples were stable. The average CT of IPC obtained from different types of samples was between 27.60 and 28.03. Common case samples obtained by the type and known results are the same. Conclusion PuriTyperTM purification kit can meet the requirements of forensic DNA testing and has important application value in forensic practice.