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目的研究甲状旁腺素相关肽(PTHrP)对骨髓基质细胞RANKL/OPG基因表达的影响。方法分离小鼠骨髓细胞后,45 ng/ml的PTHrP刺激,第6天时观察破骨细胞生成情况。90 ng/ml PTHrP刺激贴壁鼠骨髓基质细胞3 d和6 d,实时荧光PCR检测破骨细胞分化因子RANKL及骨保护素OPG基因的表达情况。结果在PTHrP的刺激下,大量破骨细胞生成。骨髓基质细胞在PTHrP的刺激下,RANKL基因表达升高,OPG基因表达降低。结论PTHrP刺激破骨细胞生成是通过上调RANKL基因表达,下调OPG基因的表达实现的。
Objective To investigate the effect of PTHrP on RANKL / OPG gene expression in bone marrow stromal cells. Methods After the mouse bone marrow cells were isolated and cultured, 45 ng / ml PTHrP was stimulated. On the 6th day, osteoclasts were observed. The adherent rat bone marrow stromal cells were stimulated with 90 ng / ml PTHrP for 3 d and 6 d, and the expression of osteoclast differentiation factor RANKL and osteoprotegerin OPG were detected by real-time fluorescence PCR. Results PTHrP stimulated a large number of osteoclasts. Bone marrow stromal cells stimulated by PTHrP, RANKL gene expression increased, OPG gene expression decreased. Conclusion PTHrP stimulates osteoclastogenesis by up-regulating RANKL gene expression and down-regulating OPG gene expression.