论文部分内容阅读
目的:观察人结直肠癌组织磷酸化哺乳动物雷帕霉素靶蛋白(phosphorylated mammalian target of rapamycin,p-mTOR)、表皮生长因子受体(EGFR)、生长因子受体结合蛋白2(Grb2)和血管内皮生长因子(VEGF)等的表达,并探讨其可能的临床意义。方法:构建含有185例结直肠癌标本的组织芯片,免疫组化法检测结直肠癌组织及癌旁组织中EGFR、Grb2、p-mTOR和VEGF的表达,并分析不同结直肠癌临床病理学特征下(如年龄、性别、浸润深度、淋巴转移、临床分期和分化程度)各自的表达情况,探讨可能的临床意义。结果:EGFR、Grb2、p-mTOR和VEGF在结直肠癌旁组织中呈少量表达或不表达,在结直肠癌组织中的表达率分别为21.1%、44.9%、42.2%和54.1%,明显高于癌旁组织(P<0.05)。结直肠癌患者不同性别、年龄、肿瘤分化程度下EGFR、Grb2、p-mTOR和VEGF表达率无统计学差异;不同浸润深度和临床分期下EGFR的表达率有统计学差异(P<0.05);不同浸润深度、淋巴转移和临床分期下p-mTOR、VEGF表达率均有统计学差异(P<0.05)。结直肠癌组织EGFR/Grb2/p-mTOR/VEGF蛋白两两间均有一定的相关性(r=0.245～0.567,P<0.05)。结论:EGFR、Grb2、p-mTOR和VEGF表达与结直肠癌的发生、发展相关,值得进一步研究以作为结直肠癌肿瘤靶向治疗新的作用靶点。
OBJECTIVE: To observe the expressions of phosphorylated mammalian target of rapamycin (p-mTOR), epidermal growth factor receptor (EGFR), growth factor receptor binding protein 2 (Grb2) Vascular endothelial growth factor (VEGF) expression, and explore its possible clinical significance. METHODS: Tissue microarrays containing 185 specimens of colorectal cancer were constructed. The expressions of EGFR, Grb2, p-mTOR and VEGF in colorectal cancer tissues and adjacent tissues were detected by immunohistochemistry. The clinicopathological features (Such as age, gender, depth of invasion, lymph node metastasis, clinical stage and degree of differentiation) of their respective expression, to explore the possible clinical significance. Results: The expressions of EGFR, Grb2, p-mTOR and VEGF in colorectal cancer tissues were little or not expressed, which were 21.1%, 44.9%, 42.2% and 54.1% respectively in colorectal cancer tissues, which were significantly higher Adjacent tissues (P <0.05). There was no significant difference in the expression rates of EGFR, Grb2, p-mTOR and VEGF between different gender, age and tumor differentiation in patients with colorectal cancer. The expression rates of EGFR in different depth of invasion and clinical stage were significantly different (P <0.05). The expressions of p-mTOR and VEGF in different depth of invasion, lymph node metastasis and clinical stage were significantly different (P <0.05). The expression of EGFR / Grb2 / p-mTOR / VEGF in colorectal cancer was correlated with each other (r = 0.245-0.567, P <0.05). CONCLUSION: The expressions of EGFR, Grb2, p-mTOR and VEGF are correlated with the occurrence and development of colorectal cancer. It is worth further studying as a new target of tumor targeting therapy for colorectal cancer.