论文部分内容阅读
目的以乙醇和高脂膳食复合因素建立大鼠脂肪肝模型。方法将雌性Wistar大鼠随机分成阴性对照组、模型组,阴性对照组饲以普通饲料,自由饮水,每天以生理盐水灌胃;模型组饲以高脂饲料,饮用15%乙醇水,每天50%乙醇溶液灌胃;实验持续5周,测定大鼠血清中甘油三酯(TG)、总胆固醇(TC)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),以及肝组织的TG、TC含量,并观察肝脏病理学变化。结果与阴性对照组比较,模型组大鼠肝指数有所升高,但差异无统计学意义(P>0.05);血清中TC明显升高[(2.93±0.62)mmol/L],与阴性对照组[(2.27±0.28)mmol/L]相比,差异有统计学意义(P<0.05);TG有所升高,但差异无统计学意义(P>0.05);肝组织中TG、TC明显升高(P<0.05);与阴性对照组比较,模型组大鼠血清中AST和ALT活力差异均无统计学意义(P>0.05);与阴性对照组比较,模型组大鼠的食物利用率明显减少(P<0.05);病理切片显示模型组肝脏发生明显的脂肪变性。结论乙醇加高脂膳食持续5周可以成功建立大鼠脂肪肝模型。
Objective To establish a fatty liver model in rats by combining ethanol and high-fat diet. Methods The female Wistar rats were randomly divided into negative control group, model group and negative control group fed with normal diet, drinking water freely and intragastrically with saline every day. The model group was fed with high-fat diet and drinking 15% ethanol water for 50% Ethanol solution for 5 weeks. The levels of triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and hepatic TG , TC content, and observe the pathological changes of liver. Results Compared with the negative control group, the liver index of the model group was increased, but the difference was not statistically significant (P> 0.05). The TC level in the serum was significantly higher than that of the negative control group [(2.93 ± 0.62) mmol / L] (2.27 ± 0.28) mmol / L], the difference was statistically significant (P <0.05); TG was increased, but the difference was not statistically significant (P> 0.05); liver tissue TG, TC was significantly (P <0.05). Compared with the negative control group, the serum AST and ALT activity in the model group had no significant difference (P> 0.05). Compared with the negative control group, the food utilization rate (P <0.05). Pathological examination revealed obvious steatosis in the model liver. Conclusion Alcohol and high fat diet for 5 weeks can successfully establish rat fatty liver model.