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我们测定了苹果幼果脱落期间磷化物含量的变化,以及脱落幼果与正常发育果实对P~(32)的吸收和转化。发现:脱落幼果中的无机磷含量较高;而其它糖磷酸醋、不稳定高能核苷酸和磷脂类等有机磷化物含量,则较发育果实降低很多。脱落幼果的ATP酶活性也比对照低。使用P~(32)进行示踪试验的结果看出,发育果实的果肉和种子中进入较多的P~(32);而脱落幼果吸收和运入的P~(32)少得多,失去了取得养分的生理优势。在脱落幼果与对照果实之间,P~(32)的转化也有明显差别。参入糖磷酸酯、高能核苷酸、磷脂、核酸和其它磷蛋白中的P~(32)显著降低。应用果肉和种子进行的试验,都得到类似结果。这些结果表明:由于脱落幼果养分供应不足,磷酸化作用水平降低,维持正常生命活动所必需的能量和原料亏缺,因此生长受阻,正常代谢作用不能进行,引起果实提前衰老,最后导致幼果脱落。
We measured the change of phospholipid content during the fall of apple young fruit, and the absorption and transformation of P ~ (32) by young and normal fruits. It was found that the content of inorganic phosphorus in the peeled off young fruit was higher than that in other fruits. However, the contents of other organic phosphides, such as other sugar phosphates, labile high-energy nucleotides and phospholipids, were much lower than those of the developed fruits. The exfoliated young fruit also had lower ATPase activity than the control. The result of tracing test using P32 showed that more P32 was found in the pulp and fruit of the developing fruit, while the P32 32 absorbed and transported into the shedding fruit was much less, Lose the physiological advantage of getting nutrients. There was also a significant difference in the transformation of P ~ (32) between young and control fruits. P (32) incorporation into sugar phosphates, high energy nucleotides, phospholipids, nucleic acids and other phosphoproteins was significantly reduced. Similar results were obtained using the tests carried out with pulp and seeds. These results indicate that due to inadequate nutrient supply, decreased phosphorylation and loss of energy and raw material needed for normal life activities, growth retardation and normal metabolism can not occur, resulting in premature aging of the fruit and eventually leading to young fruit Off.