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目的探索沙眼衣原体热休克蛋白60(cHSP60)抗体水平在女性不孕相关沙眼衣原体(Ct)感染患者中的表达及其诊断应用价值。方法将不孕年限>2年女性患者74例作为不孕组,其中原发不孕组44例,继发不孕组30例,随机选择同期妇产科门诊已生育且发现阴道分泌物异常妇女为对照组37例,采用免疫学方法检测其血清和宫颈分泌物中cHSP60,并观察其宫颈分泌物Ct病原学检查结果。结果宫颈分泌物中,不孕组Ct病原学及cHSP60免疫学检测阳性率为28.4%及40.5%均高于对照组的5.4%及13.5%,并且同组cHSP60免疫学检测检出率高于Ct病原学检出率;其中输卵管阻塞不孕患者cHSP60抗体阳性率73.7%,高于无输卵管阻塞不孕者9.09%;但血清中cHSP60抗体阳性率在不孕组与对照组,分别为12.3%和13.5%,差异无统计学意义。结论不孕组中宫颈分泌物cHSP60抗体阳性率高于对照组,且cHSP60抗体免疫学检查敏感性高于病原学检查,对女性生殖道Ct感染的筛查有更大的预诊价值,可作为女性Ct感染相关性不孕的筛查指标。
Objective To investigate the expression of cHSP60 antibody in Chlamydia trachomatis and its diagnostic value in infertile women with Ct infection. Methods 74 infertile women with infertility> 2 years as female infertility group, including 44 cases of primary infertility group and 30 cases of secondary infertility group were randomly selected, and women with abnormal vaginal discharge The control group of 37 cases, the use of immunological methods to detect serum and cervical secretions cHSP60, and observe the cervical secretions Ct etiological test results. Results The positive rates of Ct etiology and cHSP60 immunostaining in cervical secretions were 28.4% and 40.5% respectively higher than those in control group (5.4% vs 13.5%), and the detection rates of cHSP60 immunostaining in the same group were higher than those in Ct The positive rate of cHSP60 antibody in tubal obstruction infertility patients was 73.7%, which was higher than 9.09% of infertility patients without tubal obstruction; but the positive rate of cHSP60 antibody in serum was 12.3% in infertility group and control group 13.5%, the difference was not statistically significant. Conclusions The positive rate of cHSP60 antibody in cervical secretions is higher in infertility group than in control group, and the sensitivity of cHSP60 antibody immunological test is higher than that of etiological examination, which has more pre-diagnosis value for screen screening of female genital Ct, Screening of infertility related to female Ct infection.