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我们用改良的酶联免疫吸附法(ELISA),分别用牛、人天然Ⅱ型胶原和用溴化氰裂解的人Ⅱ型胶原作为包被抗原,检测了112例类风湿性关节炎患者和154例健康对照血清中抗Ⅱ型胶原的IgG型和IgA型抗体。抗天然牛Ⅱ型胶原自身抗体IgG型和IgA型阳性率RA患者组分别为19.6％和12.5％,而健康对照组为0.7％和2.0％,抗天然人Ⅱ型胶原自身抗体IgG型和IgA型阳性率RA患者分别为12.5％和16.3％,而健康对照为0％和2.5％,两类抗体的阳性率均为RA患者组显著高于对照组。用牛和人的Ⅱ型胶原作为包被抗原,其阳性率无显著性差异。这也说明了Ⅱ型胶原蛋白在种属间的抗原决定簇相似。用溴化氰裂解的人Ⅱ型胶原作为包被抗原,RA患者和健康对照组在IgG型有显著性差异,而在IgA型无统计学差异,其原因有待进一步探讨。本文根据这些结果讨论了Ⅱ型胶原自身抗体在RA发病中的意义。
We used modified enzyme-linked immunosorbent assay (ELISA), respectively, with bovine, human natural collagen type Ⅱ and human type Ⅱ collagen cleaved with cyanogen bromide as coating antigen detected in 112 patients with rheumatoid arthritis and 154 Cases of healthy control serum type II collagen anti-IgG and IgA antibodies. Anti-natural bovine type II collagen autoantibodies IgG and IgA positive rate of RA patients were 19.6% and 12.5%, while the healthy control group was 0.7% and 2.0%, anti-native human type II collagen autoantibodies IgG and IgA The positive rate of RA patients were 12.5% and 16.3%, respectively, while the healthy controls were 0% and 2.5%. The positive rates of both antibodies were significantly higher in RA patients than in controls. Cow and human type Ⅱ collagen antigen as coating, the positive rate no significant difference. This also shows that type II collagen is similar in epitopes between species. There was a significant difference in IgG type between RA patients and healthy people with type Ⅱ collagen cleaved by cyanogen bromide, but there was no statistical difference in type IgA. The reason for further discussion was needed. Based on these results, this article discusses the significance of type II collagen autoantibodies in the pathogenesis of RA.