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目的:研究迷迭香酸钠对小鼠移植性S180肉瘤的抑制作用,初步探讨其可能机制。方法:建立S180实体瘤动物模型,实验设立5组,即生理盐水对照组,迷迭香酸钠25、50、100mg/kg各剂量组、阿霉素组,腹腔注射给药10天,观察小鼠及肿瘤的生长情况,绘制小鼠体重生长曲线,计算抑瘤率及脾脏系数、胸腺系数,ELISA法检测血清中的细胞因子含量,蛋白质印迹法检测迷迭香酸钠50mg/kg组肿瘤组织中Bcl-2、Bax的表达等指标。结果:与阴性对照组相比,迷迭香酸钠能改善荷瘤小鼠的体重、精神状态等一般情况;阿霉素组和迷迭香酸钠组小鼠肿瘤体积与阴性对照组相比都有明显减小;阿霉素组抑瘤率为60%,迷迭香酸钠各剂量组抑瘤率分别33.55%、48.89%、42.22%。迷迭香酸钠的小鼠脾脏系数、胸腺系数及血清TNF-α、TNF-β、IFN-γ水平增高,迷迭香酸钠50mg/kg肿瘤组织中Bax的表达较对照组出现明显上调,Bcl-2的表达则明显下调。结论:迷迭香酸钠具有明显的抗肿瘤作用,其机制可能与提高机体免疫功能有关,同时通过调节Bcl-2和Bax来诱导肿瘤细胞凋亡。
Objective: To study the inhibitory effect of sodium rosmarinate on the transplanted S180 sarcoma in mice, and to explore its possible mechanism. Methods: The animal model of S180 solid tumor was established. Five groups were set up, namely saline control group, 25,50,100mg / kg sodium rosmarinate dosage groups, doxorubicin group and intraperitoneal injection for 10 days. The growth curves of mice and tumors were obtained. The body weight growth curves of mice were drawn. The tumor inhibition rate, spleen coefficient and thymus coefficient were calculated. The levels of cytokines in serum were detected by ELISA. The concentrations of 50 mg / kg sodium rosinate in tumor tissue In Bcl-2, Bax expression and other indicators. Results: Compared with the negative control group, sodium rosinate could improve the general condition of the tumor-bearing mice such as body weight and mental state. Compared with the negative control group, the tumor volume of the doxorubicin group and the sodium rosinate group mice The inhibition rate of doxorubicin group was 60% and that of rosmarinic acid sodium group was 33.55%, 48.89% and 42.22% respectively. The spleen coefficient, thymus coefficient, serum TNF-α, TNF-β, IFN-γ levels were increased in the mice treated with sodium rosinate, and the expression of Bax in the 50 mg / kg rosmarin sodium group was significantly higher than that in the control group Bcl-2 expression was significantly down-regulated. CONCLUSION: Sodium rosinate has obvious anti-tumor effect, and its mechanism may be related to improving immune function. At the same time, Bcl-2 and Bax can induce tumor cell apoptosis.