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目的 探索细胞因子对丙型肝炎病毒 (HCV)核心(C)基因疫苗诱生免疫应答强度的增强作用 .方法 将包含HCV- C蛋白的基因片段插入真核表达载体 pc DNA3中 ,构建重组质粒 pc DNAHCV- C.将此重组质粒单独或与包含鼠 IL-2或 IL- 12基因的表达质粒共免疫 BAL B/ c小鼠 ,EL ISA法检测 HCV C特异性抗体产生情况 ;以 pc DNAHCV- C转染小鼠 SP2 / 0细胞 ,表达 HCV C抗原者为靶细胞 ,进行 5 1 Cr释放试验 ,检测细胞毒 T淋巴细胞 (CTL s)的特异性杀伤功能 .结果 p IL - 2能够增加 pc DNAHCV- C诱导的特异性抗体的产生 ,而对 CTL的杀伤作用增强不明显 .而 p IL - 12对 pc D-NAHCV- C诱导的抗体和细胞免疫应答均有增强 ,尤以 CTL的杀伤作用增强为显著 (P<0 .0 5 ) .结论 佐以不同的细胞因子 ,可增强基因疫苗诱生的免疫应答强度 ,可能使机体对基因疫苗的免疫应答朝着有利于优先诱导 CTL 免疫应答 ,清除病原体的方向发展 .
Objective To explore the enhancement effect of cytokines on the immune response induced by hepatitis C virus (HCV) core gene (C) gene vaccine.Methods The gene fragment containing HCV-C protein was inserted into the eukaryotic expression vector pcDNA3 to construct recombinant plasmid pc DNAHCV- C. BALB / c mice were co-immunized with the recombinant plasmids either alone or with expression plasmids containing the murine IL-2 or IL-12 gene. HCV DNA-specific antibodies were detected by EL ISA assay; pcDNAHCV-C Transfection of mouse SP2 / 0 cells and expression of HCV C antigen as target cells were performed for 5 1 Cr release assay to detect cytotoxic T lymphocytes (CTLs) specific killing effect.Results p IL - 2 could increase pcDNAHCV - C induced specific antibody production, while CTL cytotoxicity was not significantly enhanced, whereas p IL - 12 enhanced the antibody and cellular immune responses induced by pc D - NAHCV - C, especially the cytotoxicity of CTL (P <0.05) Conclusion Conclusions with different cytokines can enhance the immune response induced by gene vaccines, which may make the body’s immune response to gene vaccines toward the preferential induction of CTL immune response, clear Pathogen Development.