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目的获得15个短串联重复(short tandem repeat,STR)基因座在四川汉族人群中的群体遗传学数据。方法654份血样采自四川地区无血缘关系的汉族个体。Chelex法提取DNA,PCR复合扩增,自动基因分析仪电泳,收集电泳结果数据,基因扫描分析软件计算扩增产物片段相对大小,基因分型软件进行样本基因型分型。结果全部样本的每个STR基因座都获得了清晰的基因型分型结果。15个STR基因座的基因型分布符合Hardy-Weinberg平衡。15个STR基因座的杂合度介于0.6101—0.8654之间。累计非父排除率和累计个人识别率为0.999998766和>0.999999999。结论经1次扩增电泳可获得15个STR基因座的基因型分型结果,累计非父排除率和累计个人识别率较高,适合作为四川汉族人群的遗传标记,用于人类学、疾病连锁分析、法医学亲权鉴定和个体识别等领域的研究。
Objective To obtain the population genetic data of 15 short tandem repeat (STR) loci in Sichuan Han population. Methods 654 blood samples were collected from Han unrelated Han individuals in Sichuan Province. Chelex extraction of DNA, PCR amplification, automatic gene analyzer electrophoresis, electrophoresis data collection results, gene scanning software to calculate the relative size of the amplified product fragments, genotyping software sample genotyping. Results A clear genotyping result was obtained for each STR locus in all samples. The genotypes of 15 STR loci were in accordance with Hardy-Weinberg equilibrium. The heterozygosity of the 15 STR loci ranged from 0.6101 to 0.8654. The cumulative non-parent exclusion rate and cumulative personal recognition rate are 0.999998766 and> 0.999999999. CONCLUSION: The results of genotyping of 15 STR loci obtained by one amplification electrophoresis show that the cumulative non-parent exclusion rate and cumulative individual recognition rate are high, and are suitable as genetic markers for Sichuan Han population for anthropology, disease chain Analysis, forensic paternity testing and identification of individuals in areas such as research.