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目的探讨慢性支气管炎模型的造模方法。方法24只大鼠随机分为正常组(A组)、单通道脂多糖组(B组)、双通道脂多糖组(C组)。A、B组分别给予气管内一次性注入生理盐水和脂多糖200μg,C组在B组的基础上,第2天开始,连续4d经鼻滴入脂多糖50μg.200μl-1.d-1。3周后大鼠全部处死,观察大鼠肺支气管HE染色、AB-PAS染色的病理形态学改变,并进行半定量评分。光镜下行支气管肺泡灌洗液(BALF)计数和分类,免疫组化测定肺组织黏液蛋白Muc5ac的表达,并进行半定量评分。结果与A、B组相比较,C组大鼠肺气道上皮炎性细胞浸润、纤毛粘连脱落、黏膜充血水肿等病理表现明显(P<0.05),杯状细胞增生计数增加(P>0.05)。BALF白细胞总数及中性粒细胞百分比均升高(P<0.05),Muc5ac的表达亦明显增加(P<0.05)。结论双通道给脂多糖比单通道更能建立典型的慢性支气管炎大鼠模型。
Objective To explore the method of making model of chronic bronchitis. Methods Twenty - four rats were randomly divided into normal group (group A), single - lipopolysaccharide group (group B) and double - channel lipopolysaccharide group (group C). Groups A and B were given intraperitoneal instillation of saline and lipopolysaccharide 200μg once a day, respectively. On the basis of group B, group C began to receive lipopolysaccharide 50μg.200μl-1.d-1 for 4 days continuously on the second day. After 3 weeks, all the rats were sacrificed. The pathological changes of lung bronchus of rats were observed. The morphological changes of AB-PAS staining were observed and semi-quantitatively scored. The number and classification of bronchoalveolar lavage fluid (BALF) were counted under light microscope. The expression of mucin Muc5ac in lung tissue was detected by immunohistochemistry and semi-quantitatively. Results Compared with group A and group B, the pathological manifestations of inflammatory cells such as inflammatory cell infiltration, cilia adhesion and mucosal congestion and edema in group C were significantly increased (P <0.05), and goblet cell hyperplasia count increased (P> 0.05). The total number of leucocytes and the percentage of neutrophils in BALF were increased (P <0.05), and the expression of Muc5ac was also significantly increased (P <0.05). Conclusion Dual-channel lipopolysaccharide can establish a typical rat model of chronic bronchitis more than single channel.