本实验用 MS 和 N_6培养基对春小麦4个不同品种的幼穗在离体培养条件下的反应进行了研究。发现它们在愈伤组织的诱导频率和植株的再生上,品种间有显著的差异,其中以品种欧柔最好。初步认为,在禾谷类原生质体的培养和体细胞突变体的筛选工作中,材料的基因型选择可能是成功与否的重要方面。 In this experiment, the response of four different varieties of young spikes of spring wheat in vitro culture were studied using MS and N_6 medium. It was found that there was a significant difference among the varieties in the frequency of callus induction and plant regeneration, of which the cultivar Ou Rou was the best. It is initially believed that the genotypic selection of materials may be an important aspect of success in the cultivation of cereal protoplasts and the screening of somatic mutants.