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背景:血管内皮生长因子(VEGF)是肿瘤血管再生的重要刺激因子,肿瘤血管再生与众多肿瘤患者预后密切相关。骨肉瘤血管生成与预后的关系如何?目的:研究VEGF对骨肉瘤血管生成、生长、转移及预后的影响。设计:随机对照的实验研究。地点和对象:实验地点:唐都医院全军骨肿瘤研究所。对象:69例骨肉瘤标本。干预:对69例骨肉瘤患者的临床病理资料进行回顾性研究,应用CD34,VEGF单克隆抗体对其组织切片分别进行免疫组化染色,应用地高辛标记的VEGF探针对27例原发性骨肉瘤进行原位杂交染色。分析研究VEGF表达和CD34染色阳性血管密度与患者预后关系。主要观察指标:血管内皮生长因子(VEGF)和微血管密度(MVD)。结果:VEGF阳性表达率为56.5%(39/69),VEGF阳性组肿瘤微血管计数(MVC)(35.7±9.8)及微血管周径(MVP)(2.76±0.18)μm明显高于VEGF阴性组犤MVC(7.2±2.4)及MVP(0.62±0.04)μm犦。多元回归分析显示,血管密度增高及VEGF表达阳性与骨肉瘤患者的远隔转移发生、不良预后密切相关(P<0.05)。临床病理资料中,外科手术边界微小病灶阳性情况、Enneking外科分期、肿瘤大小和患者年龄与患者预后相关(P<0.05)。患者性别、手术方法分类以及病理亚型与预后无相关性。结论:VEGF在骨肉瘤的血管生成和转移中起到重要作用,VEGF是骨肉瘤
Background: Vascular endothelial growth factor (VEGF) is an important stimulator of tumor angiogenesis. Tumor angiogenesis is closely related to the prognosis of many tumor patients. What is the relationship between angiogenesis and prognosis of osteosarcoma? Objective: To investigate the effect of VEGF on angiogenesis, growth, metastasis and prognosis of osteosarcoma. Design: Randomized controlled experimental study. Location and object: Experimental Location: Tangdu Hospital Army Bone Tumor Institute. Object: 69 cases of osteosarcoma specimens. Intervention: The clinicopathological data of 69 patients with osteosarcoma were retrospectively studied. Immunohistochemical staining was performed on the tissue sections with CD34 and VEGF monoclonal antibodies respectively. 27 patients with primary osteomyelitis Osteosarcoma in situ hybridization staining. To analyze the relationship between VEGF expression and CD34 staining positive vascular density and prognosis of patients. MAIN OUTCOME MEASURES: Vascular endothelial growth factor (VEGF) and microvessel density (MVD). Results: The positive expression rate of VEGF was 56.5% (39/69). The VEGF-positive tumor microvessel count (MVC) (35.7 ± 9.8) and microvessel circumference (MVP) (2.76 ± 0.18) μm were significantly higher than those of VEGF negative group (7.2 ± 2.4) and MVP (0.62 ± 0.04) μm 犦. Multivariate regression analysis showed that the increased vascular density and the positive expression of VEGF were closely related to distant metastasis and poor prognosis in patients with osteosarcoma (P <0.05). In clinicopathological data, the positive margins of minor lesions, Enneking surgical stage, tumor size and patient’s age were related to the prognosis of patients (P <0.05). Patient’s gender, surgical method classification and pathological subtypes had no correlation with prognosis. Conclusion: VEGF plays an important role in angiogenesis and metastasis of osteosarcoma. VEGF is osteosarcoma