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目的 探讨焦炉工外周血淋巴细胞热应激蛋白 70 (HSP70 )水平与DNA损伤的关系。方法 应用Westernblot法测定淋巴细胞HSP70水平 ,用微核分析和慧星试验 (CometAssay)测定DNA损伤程度。结果 焦炉工组淋巴细胞HSP70水平 (196 0± 5 36 )高于对照组 (1712± 44 2 ) ,差异有显著性(P <0 .0 5 ) ;焦炉工组微核率 (5 16 2 /10 6± 2 2 0 5 /10 6)和DNA损伤积分 (6 70 0 0 /10 6± 6 4934/10 6)也都明显高于对照组 (2 36 2 /10 6± 12 41/10 6、2 8936 /10 6± 30 874/10 6) ,差异有显著性 (P <0 .0 5 )。焦炉工人组中淋巴细胞HSP70水平与微核率和DNA损伤积分均呈明显的负相关 ,且差异有显著性 (r值分别为- 0 .46 1、- 0 .6 6 3,均P <0 .0 1)。结论 焦炉工外周血淋巴细胞HSP70水平的增高可能与保护DNA免受致癌物损伤有关。
Objective To investigate the relationship between heat stress protein 70 (HSP70) in peripheral blood lymphocytes and DNA damage in coke oven workers. Methods Western blotting was used to determine the level of HSP70 in lymphocytes. The degree of DNA damage was determined by micronucleus test and Comet Assay. Results The HSP70 level in the coke oven workers was higher than that in the control group (196 ± 5 36) (1712 ± 44 2) (P <0.05) 2/10 6 ± 2 2 05/10 6) and DNA damage scores (6 70 0 0 10 6 ± 6 4934/10 6) were also significantly higher than those in the control group (2 36 2/10 6 ± 12 41 / 10 6,2 8936/10 6 ± 30 874/10 6), the difference was significant (P <0.05). The level of HSP70 in lymphocyte of coke oven workers group was negatively correlated with micronucleus rate and DNA damage scores (r = -0.461, -0.663, P < 0 .0 1). Conclusion The increase of HSP70 in peripheral blood lymphocytes of coke oven workers may be related to the protection of DNA from carcinogen damage.