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取花魔芋(AmorphophalusKonjacK.Koch)长1.5cm的不定芽在附加1mg/L6-BA和0.05~0.1mg/LNAA的MS-NH+4培养基上,4℃黑暗保存180天后,存活达100%。不定芽在低温保存过程中,过氧化物酶活性升高,可溶性糖含量增加。培养基添加甘露醇后,过氧化物酶活性升高幅度变小,而可溶性糖含量进一步增加,保存后的花魔芋不定芽能快速恢复生长。甘露醇有利于芽的低温保存。
Adventitious buds of Amorphophalus Konjac (Koch) with a length of 1.5 cm were cultured on MS-NH + 4 medium supplemented with 1 mg / L 6-BA and 0.05-0.1 mg / L NAA and survived for 100 days at 4 ° C in the dark %. Adventitious buds stored at low temperature, peroxidase activity increased soluble sugar content increased. When mannitol was added into the medium, the increase of peroxidase activity was smaller and the content of soluble sugar was further increased. The adventitious buds of Amorphophallus konjac could be rapidly restored after being preserved. Mannitol is conducive to the preservation of buds at low temperature.