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本文建立了试管内软琼脂小鼠L_(1210)、P_(388)细胞集落形成法,并与平皿和24孔板软琼脂集落形成法进行了比较,肯定了试管法具有用肉眼计集落数、简便、快速、经济、污染率低,用药量少等优点.本法的集落形成率(PE)平均为0.67±0.17.用本法评价了阿霉素等5种临床抗癌药对抗L_(1210)、P_(388)细胞的作用,其剂量-反应曲线呈指数性直线.用IC_(50)(μg/ml)表示抗肿瘤效应,以阿霉素和氮芥作用最强,其次顺铂、卡氮芥和三尖杉酯碱作用最弱.试管法与平皿法的结果一致.
In this study, soft agar mouse L_ (1210), P_ (388) colony forming assay was established in vitro and compared with plate and 24-well plate soft agar colony forming method. Simple, rapid, economical, low pollution rate, less dosage, etc. The average colony forming rate (PE) of this method is 0.67 ± 0.17.The efficacy of five clinical anticancer drugs such as doxorubicin against L 1210 ), P388 cells, the dose-response curve showed an exponential straight line.The anti-tumor effect was expressed with IC 50 (μg / ml), the strongest was doxorubicin and nitrogen mustard, followed by cisplatin, Carmustine and harringtonine had the weakest effect, and the results of tube method and plate method were the same.