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目的研究沙林对猴全血乙酰胆碱酯酶(AChE)的抑制,磷酰化酶的老化速率及肟类药物的重活化作用。方法 5,5-连硫代-双-2-硝基苯甲酸(DTNB)法测定AChE活性。结果沙林抑制猴全血AChE的IC50值为6.3×10-8mol/L,IC90值为1.5×10-7mol/L。在0.1、0.33和1 mmol/L的浓度下,氯解磷定对沙林抑制猴全血AChE的重化活率分别为16%、45%和83%;双复磷对沙林抑制猴全血AChE的重化活率分别为18%、39%和70%;碘解磷定对沙林抑制猴全血AChE的重化活率分别为14%、41%和72%。采用1 mmol/L的氯解磷定为重活化剂时,沙林抑制猴全血AChE磷酰化酶的半老化时间约为12 h。结论沙林中毒后应尽早使用氯解磷定等肟类重活化剂。在低浓度0.1 mmol/L时,双复磷重活化效果最好;随着不同重活化剂浓度的增加,氯解磷定显示出较好的重活化效果。氯解磷定为重活化剂时,急性中毒症状控制后,建议应在12 h内重复使用。
Objective To study the inhibition of sarin on the monocyte chemoattractant AChE, the aging rate of phosphorylase and the reactivation of oximes. Methods 5,5-dithiobis-2-nitrobenzoic acid (DTNB) method was used to determine AChE activity. Results IC50 value of sarin inhibition whole blood AChE was 6.3 × 10-8mol / L, IC90 value was 1.5 × 10-7mol / L. Under the concentrations of 0.1, 0.33 and 1 mmol / L, the reactivation rates of sarin against whole blood AChE were 16%, 45% and 83%, respectively; The activity of AChE in blood was 18%, 39% and 70%, respectively. The rates of resorption of sarin against AChE were 14%, 41% and 72%, respectively. With 1 mmol / L of chlorophyll-phosphate as a reactivator, the half-aging time of Sarin inhibition of whole blood AChE phosphorylase was about 12 h. Conclusion Sarin poisoning should be used as soon as possible after chlorophenanthrene and other oxime reactivation agent. At the low concentration of 0.1 mmol / L, double-reactivation was the best. With the increase of different reactivating agents, chlorpromazine showed a better reactivation effect. Chlorine phosphate as a reactivator, the symptoms of acute poisoning control, it is recommended should be repeated within 12 h.