论文部分内容阅读
[目的]探讨全反式维甲酸(RA)与甘草酸(GL)和18β鄄甘草次酸(GA)对高转移人肺癌细胞(PGCL3)增殖抑制和抗侵袭的作用及其联合作用。[方法]用2.5μmol/L和5.0μmol/LRA、0.5mmol/L和1.0mmol/LGL、25μmol/L和50μmol/LGA,以及5.0μmol/LRA与0.5mmol/LGL联合用药和5.0μmol/LRA与25μmol/LGA联合用药处理PGCL3细胞96h,进行细胞增殖抑制试验、软琼脂集落形成试验、侵袭、运动和粘附试验,以及组织蛋白酶B活性的测定,观察药物对PGCL3细胞增殖和侵袭能力的影响。[结果]RA、GL和GA可减弱PGCL3细胞增殖能力和侵袭能力(P<0.05和P<0.01),呈剂量依赖性,半增殖抑制浓度(IC50)分别为12.6μmol/L、1.8mmol/L和145.3μmol/L。RA与GL联合作用及RA与GA联合作用对细胞侵袭抑制率均大于两药单独作用之和,呈协同作用。GL和GA对细胞的运动、粘附、组织蛋白酶B分泌和软琼脂集落形成均有显著抑制作用(P<0.05和P<0.01)。[结论]RA、GL和GA均有抗PGCL3人肺癌细胞增殖和侵袭作用,RA与GL或GA联合作用均有协同抗侵袭作用,GL和GA抗侵袭机理是对侵袭过程中多个关键环节起抑制作用。
[Objective] To investigate the effects of all-trans retinoic acid (RA) combined with glycyrrhizic acid (GL) and 18βglycyrrhetinic acid (GA) on the proliferation inhibition and anti-invasion of highly metastatic human lung cancer cells (PGCL3) and their combined effects. [Method] 2.5μmol/L and 5.0μmol/LRA, 0.5mmol/L and 1.0mmol/LGL, 25μmol/L and 50μmol/LGA, and 5.0μmol/LRA combined with 0.5mmol/LGL and 5.0μmol/L LA were used. PGCL3 cells were treated with 25μmol/LGA for 96 hours. The cell proliferation inhibition assay, soft agar colony formation assay, invasion, movement and adhesion assays, and the activity of cathepsin B were measured. The effects of drugs on the proliferation and invasion of PGCL3 cells were observed. [Results] RA, GL and GA could weaken the proliferation and invasion ability of PGCL3 cells (P<0.05 and P<0.01) in a dose-dependent manner. The half-inhibition inhibitory concentration (IC50) was 12.6 μmol/L and 1.8 mmol/L, respectively. And 145.3μmol/L. The combined effect of RA and GL and the combined effect of RA and GA on cell invasion inhibition rate were greater than the sum of the effects of the two drugs alone, showing a synergistic effect. GL and GA significantly inhibited cell movement, adhesion, cathepsin B secretion, and soft agar colony formation (P<0.05 and P<0.01). [Conclusion] RA, GL and GA all have anti-PGCL3 human lung cancer cell proliferation and invasion. RA, GL or GA have synergistic anti-invasion effect. GL and GA anti-invasion mechanism is the key link in the invasion process. Inhibition.