论文部分内容阅读
恶性实体肿瘤的转移是对患者生命的最大威胁。根据肿瘤细胞异质性的理论,将人肺区细胞癌细胞系(PLA-801)克隆化后建立了两株分别具有高低转移能力的克隆化细胞株(PLA-801D和PLA-801C),成为研究转移机理的良好模型。层粘连蛋白是细胞外间质的重要成份之一,具有影响细胞的粘附,移动和修复等功能,被称为附着因子,在恶性肿瘤的侵袭和转移中起重要作用。我们对这两克隆化株瘤细胞的超微结构进行了观察,并利用免疫胶体金技术对层粘连蛋白在瘤细胞上的分布进行了研究。结果表明,高转移性的PLA-801D株瘤细胞表面有较为丰富的微绒毛,细而且长,在瘤细胞之闻未见到明确的细胞问连接结构[图1]。相反,在低转移性的PLA-801C株瘤细胞中,细胞表面较光滑,微绒毛
The metastasis of malignant solid tumors is the greatest threat to the lives of patients. According to the theory of tumor cell heterogeneity, two cloned cell lines (PLA-801D and PLA-801C) with high and low metastasis capacity were established after the human lung cell carcinoma cell line (PLA-801) was cloned. Study the good model of the transfer mechanism. Laminin is one of the important components of the extracellular matrix. It has the functions of affecting cell adhesion, migration and repair. It is called an attachment factor and plays an important role in the invasion and metastasis of malignant tumors. We observed the ultrastructure of these two cloned tumor cells and used immunocolloid gold technology to study the distribution of laminin on tumor cells. The results showed that the tumor cells on the surface of the highly metastatic PLA-801D had abundant microvilli, fine and long, and no clear intercellular connection structure was observed in the tumor cells [Fig. 1]. In contrast, in the low metastatic PLA-801C strains, the cell surface is relatively smooth, microvilli