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目的 :采用流式细胞术分析胆脂瘤上皮细胞的 DNA特征 ,探讨胆脂瘤的病理学属性。方法 :利用机械捣碎法将新鲜的胆脂瘤和外耳道上皮制成单细胞悬液 ;采用 PI法 ,对 2 5例胆脂瘤上皮和 10例正常外耳道细胞上皮的 DNA进行流式细胞术分析。结果 :胆脂瘤上皮细胞和外耳道上皮细胞的核型均为二倍体 ,未见异倍体。胆脂瘤上皮细胞 S期细胞数 (10 .9± 2 .32 ) %明显高于外耳道上皮 (7.31± 2 .82 ) %。结论 :虽然胆脂瘤破坏力强 ,易复发 ,但从染色体水平看来 ,仍提示为一种炎性病变 ,未见有恶变倾向。
Objective: To analyze the DNA characteristics of cholesteatoma epithelial cells by flow cytometry and explore the pathological properties of cholesteatoma. Methods: Fresh cholesteatoma and external auditory meatus epithelium were made into single cell suspension by mechanical masking method. The DNA of 25 cases of cholesteatoma epithelium and 10 cases of normal external auditory canal epithelial cells was analyzed by flow cytometry . Results: The karyotypes of cholesteatoma epithelial cells and external auditory canal epithelial cells were diploid with no aneuploidy. The number of S-phase cells in cholesteatoma epithelial cells (10.9 ± 2.32)% was significantly higher than that of the external auditory canal epithelium (7.31 ± 2.82%). CONCLUSIONS: Although cholesteatoma is highly destructive and prone to relapse, it appears to be an inflammatory lesion at chromosome level, with no malignant tendency.